NMR-based Metabolomics Laboratory
NMLab, the NMR-based Metabolomics Laboratory of Sapienza University of Rome, is part of the Sapienza Research Infrastructure (SRI). It is an integrated system of instruments and expertise primarily dedicated to metabolomics analysis. Its core facility is the high-resolution NMR spectrometer JNM-ECZ 600R, equipped with a 14.09 Tesla magnet, a sample changer and a cryoprobe.
The spectrometer is hosted in the Department of Chemistry, on the ground floor of the Cannizzaro Building (CU014), while the sample preparation laboratories are located on the second floor of the same building.
The research areas include clinical and biomedical sciences, agri-food and nutrition, technological-industrial applications and sustainability, with impact on human and animal health; precision medicine, pharmacology and nutrition; the agri-food sector, functional foods and nutritional quality; as well as production processes, innovation and agro-industrial sustainability.
The laboratory provides the equipment and expertise necessary for sample preparation for metabolomics analysis, spectra acquisition, data processing and data analysis.
The features of the JNM-ECZ 600R spectrometer do not limit its application to metabolomics alone. They also allow its use in many other research fields such as molecular structure determination, mixture composition analysis and any application requiring high resolution NMR spectra. The spectrometer supports one- and two-dimensional measurements on a wide range of nuclei. It is equipped with a 30 position autosampler refrigerated down to -20°C to meet high-throughput analysis requirements typical of metabolomics, and with a cryoprobe for experiments requiring high sensitivity, especially useful for nuclei such as 13C and 31P.